Alarelin Acetate is a synthetic GnRH agonist which can directly inhibit proliferation and DNA synthesis of rat GSMC through GnRH receptors.

在alarelin存在下的细胞活性显著低于alarelin缺失情况下的活性。当浓度达到10-5 M时，对细胞活性的刺激效果得以实现，并且其作用呈剂量依赖性[1]。

Alarelin能通过直接作用于大鼠的壁细胞以及抑制迷走神经功能来抑制胃酸分泌[2]。同时，Alarelin可显著提高大鼠GSMC的G1期比例，并降低S期比例[1]。

The cells are trypsinized in a solution of 2.5 g/L trypsin and seeded in a 96-well plate. After the cells are grown for 24 h to approximately 800 g/L subconfluent state, 0.1 mL medium containing 2.5% calf serum and various concentrations (0.001, 0.1, 10 μM) of alarelin is added to each well, respectively, and incubated for 24 h in a CO2 incubator. Each concentration is tested in at least 12 wells. Briefly, 15 μL of MTT solution is added to each well and incubated for 4 h. Then, the medium and MTT are removed and 150 μL of DMSO is added to each well and shaken for 10 min to dissolve the crystal. The OD is determined at 490 nm using an ELISA reader[1].